Circular Dichroism (CD)

Circular Dichroism (CD) is a spectroscopic technique used to study the chiral properties of molecules.

Here are its key aspects:Principle: CD measures the differential absorption of left-handed and right-handed circularly polarized light by chiral substances, providing information about their secondary and tertiary structures.

Applications: Commonly used to analyze protein folding, conformational changes, and interactions. It’s also applied in studying nucleic acids and other biomolecules.Data: Provides insights into secondary structure elements like α-helices and β-sheets in proteins, and can detect structural changes in response to environmental conditions.

 

Instrumentation: Typically involves a CD spectrometer that measures the intensity of light absorbed by the sample at various wavelengths.

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